>> GD 641 REAL QUALI MICRO
Device for the research of bacterial contamination in blood plateletCode GD 641
Destination for use. Real Quali Micro Kit, is an in vitro qualitative test for a quick detenction of the bacterial contamination in blood platelet, through the Real Time PCR tecnique (Polymerase Chain Reaction).
Informations obteneid through the test, in addition to other kind of laboratory’s analysis (for example the culture of bacteria), allows to determine this contamination.
info:
The target.
The 16S r-DNA region of bacterial genome is particularly indicated for the target selection, because it is well conserved for a lot of bacteria. The device uses two primers 16S rev and 16S fwd, which define a 466 bp area in this region (16S region of bacterial genome); the complementary region of the two primers is represented for the most by known bacteria, so it guarantees the analytical specificity. The amplification’s reaction is executed (upon the inactivation of the restriction’s enzyme) by a thermostable TAQ-DNA polymerase that, with the presence of cofactor Magnesium Chloride and with the right plug which contains dUTPs/ dNTPs, warrant an high emivita and an homogeneous efficiency during all the analytical process. In the mix of deoxynucleosidi trifosfati, the deoxitimidina is substituted with the deoxyuracile, this substitution allows the utilization of Uracil DNA glicosilasi in order to check the carry over.
The 16S r-DNA region of bacterial genome is particularly indicated for the target selection, because it is well conserved for a lot of bacteria. The device uses two primers 16S rev and 16S fwd, which define a 466 bp area in this region (16S region of bacterial genome); the complementary region of the two primers is represented for the most by known bacteria, so it guarantees the analytical specificity. The amplification’s reaction is executed (upon the inactivation of the restriction’s enzyme) by a thermostable TAQ-DNA polymerase that, with the presence of cofactor Magnesium Chloride and with the right plug which contains dUTPs/ dNTPs, warrant an high emivita and an homogeneous efficiency during all the analytical process. In the mix of deoxynucleosidi trifosfati, the deoxitimidina is substituted with the deoxyuracile, this substitution allows the utilization of Uracil DNA glicosilasi in order to check the carry over.
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Date release: 09/03/2008
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